Estradiol (serum)
Estradiol (E2) varies by sex, menstrual cycle phase, pregnancy, and assay method. The ranges shown include two published pregnancy reference sets used in clinical laboratories.
| Category | Value 1 | Value 2 | Value 3 |
|---|---|---|---|
| Prepubertal children | < 10 pg/mL | ||
| Male | < 60 pg/mL | ||
| Females ovulating — phase | Early follicular | Late follicular | Luteal |
| pg/mL | 30 – 100 | 100 – 400 | 60 – 150 |
| Pregnant (Set 1) Abbassi-Ghanavati et al. |
1st Trimester 188 – 2,497 pg/mL |
2nd Trimester 1,278 – 7,192 pg/mL |
3rd Trimester 6,137 – 3,460 pg/mL* |
| Pregnant (Set 2) Roche Diagnostics E2 III |
154 – 3,243 pg/mL | 1,561 – 21,280 pg/mL | 8,525 – >30,000 pg/mL |
| Postmenopausal | < 18 pg/mL |
* Published as given in original table; third-trimester ranges vary by assay and laboratory.
References
- Abbassi-Ghanavati M, Greer LG, Cunningham FG. Pregnancy and laboratory studies: a reference table for clinicians. Obstet Gynecol. 2009;114:1326–31. PMID: 19935037.
- Roche Diagnostics Estradiol III assay. University of Iowa Diagnostic Laboratories. Test Handbook. Accessed 11/26/2025.